Escherichia coli engineering bacterium and method for whole-cell catalytic production of steviol

• Main Authors: WEN MANMAN, WEI YUTUO, LAN QING, PANG HAO, ZHOU JIE, DU LIQIN, HUANG RIBO
• Format: Patent
• Language: Chinese; English
• Published: 13.12.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a recombinant escherichia coli engineering bacterium pSE-sbgl-spbgl1 and a method for whole-cell catalytic production of steviol by the recombinant escherichia coli engineeringbacterium pSE-sbgl-spbgl1. The strain co-expresses double beta-glucosidase SPBGL1 and SBGL in a polycistron form, and with a stevia rebaudiana crude extract as a substrate, stevioside and rubusosidein the stevia rebaudiana crude extract are catalyzed to be hydrolyzed to produce steviol by using the whole-cell catalysis method. The engineering bacterium pSE-sbgl-spbgl1 is used for catalyzing stevioside to produce steviol in a whole-cell mode, and the method has the advantages of being high in conversion rate, high in yield, single in product, low in energy consumption, short in consumed time,easy to operate, high in industrialization potential and the like. 本发明公开了一株重组大肠杆菌工程菌pSE-sbgl-spbgl1及利用该菌株全细胞催化生产甜菊醇的方法，该菌株通过多顺反子形式共表达双β-葡萄糖苷酶SPBGL1和SBGL，用全细胞催化的方法，以甜叶菊粗提物为底物，催化其中的甜菊苷和甜茶苷水解生产甜菊醇。本发明利用工程菌pSE-sbgl-spbgl1全细胞催化甜菊糖生