Saline-alkali-resistant aspergillus flavus as well as separation method and application thereof

• Main Authors: TAO RUNZHI, ZHANG SENLIN, FENG ZEJUAN
• Format: Patent
• Language: Chinese; English
• Published: 13.12.2019
• Subjects: ADHESIVES; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DYES; ENZYMOLOGY; MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MISCELLANEOUS APPLICATIONS OF MATERIALS; MISCELLANEOUS COMPOSITIONS; MUTATION OR GENETIC ENGINEERING; NATURAL RESINS; PAINTS; POLISHES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses saline-alkali-resistant aspergillus flavus as well as a separation method and an application thereof. The method comprises the following steps: coating a PDA culture medium solid flat plate containing 250.0 g/L of NaCl with different concentrations with a straw powder suspension subjected to gradient dilution; treating and repeating for three times, culturing for 4-15 days,picking well-grown single colonies, and inoculating the single colonies onto a screening culture medium CMC-Na solid culture medium to generate transparent ring-shaped strains so as to obtain aspergillus flavus with a preservation name of aspergillus flavus W3 and a preservation number of CGMCC NO.17973. The aspergillus flavus strain disclosed by the invention can degrade straws in a high-saline-alkali environment, the straw degradation capacity is high in a high-saline-alkali environment, the strain can be directly used for decomposing straw fermentation and other biological substances in the field, the capacity of