Penicillium chrysogenum with high salt and alkali resistance as well as separation method and application thereof

• Main Authors: TAO RUNZHI, ZHANG SENLIN, FENG ZEJUAN
• Format: Patent
• Language: Chinese; English
• Published: 13.12.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DISPOSAL OF SOLID WASTE; ENZYMOLOGY; FERTILISERS; MANUFACTURE THEREOF; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE; PERFORMING OPERATIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECLAMATION OF CONTAMINATED SOIL; RECLAMATION OF CONTAMINED SOIL SOIL; SPIRITS; TRANSPORTING; VINEGAR; WINE

The invention discloses penicillium chrysogenum with high salt and alkali resistance as well as a separation method and an application thereof. The method comprises the following steps: coating a cornstraw powder culture medium solid flat plate containing 250.0 g/L NaCl with different concentrations with a straw powder suspension subjected to gradient dilution, treating and repeating for 3 times,culturing for 4-15 days, picking well-grown single colonies, inoculating the single colonies onto a screening culture medium CMC-Na solid culture medium, and generating a transparent ring-shaped strain to obtain penicillium chrysogenum with a preservation name of penicillium chrysogenum W2 and a preservation number of CGMCC NO. 17972. The penicillium chrysogenum with high saline-alkali resistancehas high impermeability, is beneficial to improving the degradation capacity of straws in a high saline-alkali environment, can also be directly used for decomposing straw fermentation and other biological substances in fields