Immunoblotting-based method for identifying leather

• Main Authors: YANG HAILIANG, SHANG YATING, ZHENG HAORAN, ZHAI YUJIE, PENG ZHIQIN, WANG BING
• Format: Patent
• Language: Chinese; English
• Published: 06.12.2019
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to the technical field of leather identification, and discloses an immunoblotting-based method for identifying leather. The method comprises the steps of extracting proteins in the leather by using an alkaline method; then screening the proteins through a deer protein standard sequence to obtain characteristic peptide fragments; then obtaining a protein sequence antibody through immune response; analyzing the protein sequence antibody by using a western blot technology to investigate whether the sample leather is deerskin or not. The method is environment-friendly and pollution-free. The immunoblotting-based method for identifying leather has the advantages that the required amount for sample measurement is less, the method is not influenced by adverse factors such assurface damage or degradation of the tested sample and the problem of counterfeiting in animal leather can be accurately and efficiently solved. 本发明涉及皮革鉴定技术领域，公开了一种基于免疫印迹法鉴别皮革的方法，本发明采用碱法提取皮革中的蛋白，然后通过鹿蛋白标准序列筛选，得到了特征肽段，接着通过免疫反