METHOD FOR PRODUCING RNA

• Main Authors: MITSUHASHI MAYU, KRYLOV ALEKSANDR ALEKSANDROVICH, LOBANOVA YULIYA SERGEEVNA, HASHIRO SHUHEI, MASHKO SERGEI VLADIMIROVICH, YASUEDA HISASHI
• Format: Patent
• Language: Chinese; English
• Published: 22.11.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells. 提供了一种生产RNA的方法。通过在培养基中培养具有目标RNA的表达单元的棒状杆菌细菌以表达所述目标RNA并且在所述细菌细胞中积累所述目标RNA，并且从所述细胞中收集所述目标RNA来生产目标RNA，所述棒状杆菌细菌已经被修饰而使得核糖核酸酶III的活性降低。