Liquid chromatography-mass spectrum-based traditional Chinese medicine curing patch effective component detecting and analyzing method

• Main Authors: GUAN WENNA, MAO XINTAO
• Format: Patent
• Language: Chinese; English
• Published: 19.11.2019
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a liquid chromatography-mass spectrum-based traditional Chinese medicine curing patch effective component detecting and analyzing method and belongs to the traditional Chinesemedicine component detection technical field. According to the method, a liquid chromatography-tandem triple quadrupole mass spectrometer is adopted to establish a quantification method for chlorogenic acid, syringin, rutin, calycosin, quercetin, luteolin, kaempferol, Hispidulin and cnidium lactone in a traditional Chinese medicine curing patch; and an instrument analysis method is optimized by means of the standard substances of the nine key effective components. According to the method disclosed by the invention, the instrument analysis method is optimized by means of the standard substancesof the nine key effective components; and the optimization of the instrument analysis method involves liquid chromatography condition optimization and mass spectrum detection condition and mass spectrum parameter optimization