REGIOSELECTIVE HYDROXYLATION OF ISOPHORONE AND FURTHER CONVERSION TO KETOISOPHORONE

• Main Authors: BONRATH WERNER, SCHURMANN MARTIN, CASSTELLANOS JOSE RUBEN, TURNER NICHOLAS, MATTEVI ANDREA, TAVANTI MICHELE, PARMEGGIANI FABIO
• Format: Patent
• Language: Chinese; English
• Published: 15.11.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

The present invention relates to novel process for the production of ketoisophorone via biocatalytic conversion of isophorone, in particular a one-pot biocatalytic system for conversion of alpha-isophorone in a two-step oxidation process, with a first oxidation being catalyzed by a heme containing oxidoreductase such as a cytochrome P450 monooxygenase followed by another oxidation which can be either a chemical reaction or a biocatalytic reaction, in particular wherein the oxidation is catalyzed by an NAD(P) or NADP(H)-dependent oxidoreductase. The invention further provides polypeptides and nucleic acid sequences coding for cytochrome P450 monooxygenase with modified (higher) substrate selectivity, total turnover numbers and/or (re)activity compared to the wild-type enzyme. Ketoisophoroneis useful as building block in the synthesis of vitamins and carotenoids. 本发明涉及经由异佛尔酮的生物催化转换来产生氧代异佛尔酮的新方法，特别是用于在两步氧化方法中转换α-异佛尔酮的一锅生物催化体系，其中第一氧化由含有血红素的氧化还原酶(诸如细胞色素P450单加氧酶)催化，之后是另一氧化，所述另一氧化可以是化学反应或生物催化反应，特别地其中所述氧化由NAD(P)或NADP