POLYNUCLEOTIDE ADAPTERS AND METHODS OF USE THEREOF

• Main Authors: ANDERSEN MARK, ALLEN MICHAEL
• Format: Patent
• Language: Chinese; English
• Published: 12.11.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Provided are methods and compositions for reducing unfavorable dimer formation and thereby improving library preparation, e.g., for sequencing. Compositions and methods include adapters comprising extensive 5' overhang sequences and blunt end or T overhang 3' sequences to ends of target nucleic acid(s) to facilitate amplification and analysis of such sequences. 提供了用于减少不利的二聚体形成并由此改善文库制备(例如用于测序)的方法和组合物。组合物和方法包括衔接子，其包含延伸的5'突出端序列和平端或T突出端3'序列至靶核酸的末端，以促进这些序列的扩增和分析。