POLYNUCLEOTIDE ADAPTERS AND METHODS OF USE THEREOF

Provided are methods and compositions for reducing unfavorable dimer formation and thereby improving library preparation, e.g., for sequencing. Compositions and methods include adapters comprising extensive 5' overhang sequences and blunt end or T overhang 3' sequences to ends of target nu...

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Bibliographic Details
Main Authors ANDERSEN MARK, ALLEN MICHAEL
Format Patent
LanguageChinese
English
Published 12.11.2019
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:Provided are methods and compositions for reducing unfavorable dimer formation and thereby improving library preparation, e.g., for sequencing. Compositions and methods include adapters comprising extensive 5' overhang sequences and blunt end or T overhang 3' sequences to ends of target nucleic acid(s) to facilitate amplification and analysis of such sequences. 提供了用于减少不利的二聚体形成并由此改善文库制备(例如用于测序)的方法和组合物。组合物和方法包括衔接子，其包含延伸的5'突出端序列和平端或T突出端3'序列至靶核酸的末端，以促进这些序列的扩增和分析。
Bibliography:Application Number: CN201880020535