HPLC characteristic chromatogram determination method of xiebai powder substance standard
The invention provides an HPLC characteristic chromatogram determination method of a xiebai powder substance standard, and belongs to the technical field of traditional Chinese medicines. The method comprises the following steps that (1) a test product solution is prepared; (2) a reference substance...
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Main Authors | , , , |
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Format | Patent |
Language | Chinese English |
Published |
08.11.2019
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Subjects | |
Online Access | Get full text |
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Summary: | The invention provides an HPLC characteristic chromatogram determination method of a xiebai powder substance standard, and belongs to the technical field of traditional Chinese medicines. The method comprises the following steps that (1) a test product solution is prepared; (2) a reference substance solution is prepared; (3) chromatographic conditions are set, wherein octadecyl silane bonded silica gel serves as a filler, acetonitrile serves as a mobile phase A, a 0.02-0.1% phosphoric acid solution serves as a mobile phase B, gradient elution is conducted, the detection wavelength is 224-320 nm, the flow rate is 0.8-1.2 ml/min, and the column temperature is 25-35 DEG C; (4) determination is conducted, wherein 5-20 microliters of the reference substance solution and 5-20 microliters of thetest product solution are precisely sucked respectively and injected into a liquid chromatograph for determination, and a chromatogram is recorded. The method can comprehensively and accurately evaluate the overall and inhere |
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Bibliography: | Application Number: CN201910812642 |