HPLC characteristic chromatogram determination method of xiebai powder substance standard

• Main Authors: CHEN ZHIJIE, YE SUMEI, LIU YUJUN, ZHAO MEILING
• Format: Patent
• Language: Chinese; English
• Published: 08.11.2019
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides an HPLC characteristic chromatogram determination method of a xiebai powder substance standard, and belongs to the technical field of traditional Chinese medicines. The method comprises the following steps that (1) a test product solution is prepared; (2) a reference substance solution is prepared; (3) chromatographic conditions are set, wherein octadecyl silane bonded silica gel serves as a filler, acetonitrile serves as a mobile phase A, a 0.02-0.1% phosphoric acid solution serves as a mobile phase B, gradient elution is conducted, the detection wavelength is 224-320 nm, the flow rate is 0.8-1.2 ml/min, and the column temperature is 25-35 DEG C; (4) determination is conducted, wherein 5-20 microliters of the reference substance solution and 5-20 microliters of thetest product solution are precisely sucked respectively and injected into a liquid chromatograph for determination, and a chromatogram is recorded. The method can comprehensively and accurately evaluate the overall and inhere