Mouse esophageal cancer model and construction method thereof

• Main Authors: CHEN YANWEN, HUANG TUXIONG, FU LI
• Format: Patent
• Language: Chinese; English
• Published: 08.11.2019
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to the technical field of biomedicine, in particular to a mouse esophageal cancer model and a construction method thereof. The construction method includes: inducing mice by a chemical carcinogenic agent to construct a primary mouse esophageal cancer model to obtain an esophageal cancer in-situ tumor tissue; transplanting the esophageal cancer in-situ tumor tissue to naked mice subcutaneously, and performing in-vivo culture to obtain a transplanted tumor tissue; sequentially subjecting the transplanted tumor tissue to removal of part of fiber cells according to a rapid adherence method, enriching of epithelial tumor cells according to an epithelial culture method and removal of residual fiber cells according to a rapid digestion method, and separating to obtain the mouse esophageal cancer model. The mouse esophageal cancer model used for rapid construction of perfectly-immune mouse esophageal cancer model is constructed, and the stable and reliable mouse esophageal cancer model construct