Two-time eight-layer embedding method of freeze-dried powder of lactic acid bacteria

• Main Authors: LI CUIHUA, SI SHUFENG, SUN CHUAN, CAO WEICHAO, WANG WEIXIN, PAN YULIN, MA MINGLEI, ZHANG YONGJUAN
• Format: Patent
• Language: Chinese; English
• Published: 22.10.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of lactic acid bacteria, in particular to a two-time eight-layer embedding technology for improving freeze-drying survival rate and protecting biological activity of lactic acid bacteria, in particular to a two-time eight-layer embedding method of freeze-dried powder of lactic acid bacteria. The two-time eight-layer embedding method comprises the stepsof adding sterilized chitosan into a fermentation liquor at the fermentation end point of lactic acid bacteria, executing even stirring, then executing centrifugation to obtain centrifuged bacterial sludge, and adding a freeze-drying protective additive accounting for 15.0-35.0% of the weight of the bacterial sludge into the centrifuged bacterial sludge for freeze-drying, atomizing pullulan polysaccharide, and spray-drying bacterial powder. By adopting the two-time eight-layer embedding method, the death rate of the bacteria in the freeze-drying process is reduced, the activity of the freeze-dried bacterial powder is