Method for delaying pupation of domestic silkworms

• Main Authors: WU XIAOFENG, ZHANG JIANJIA, CHEN NAN
• Format: Patent
• Language: Chinese; English
• Published: 18.10.2019
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a method for delaying pupation of domestic silkworms. The method utilizes ecdysteroid UDP-glucosyltransferase (EGT) expressed by BmNPV code by Bombyx mori nuclear polyhedrosisvirus added in mulberry leaves, the ecdysone in the silkworm is inactivated, thereby pupation of domestic silkworm is delayed. The principle is that the EGT enzyme can catalyze the reaction of transferring a glycosyl group in UDP-glucose to ecdysone to inactivate the ecdyson, so that the ecdysis and pupation of the silkworm are blocked. The method solves the problem that the fresh cocoon must be dried immediately to kill the silkworm chrysalis in the silkworm cocoons, the production cost is saved, and the quality of silk is ensured, which creates conditions for realizing fresh silk and improving production efficiency. 本发明公开了一种延迟家蚕化蛹的方法。本发明利用在桑叶中添加家蚕核型多角体病毒BmNPV编码表达的蜕皮甾醇尿苷二磷酸葡萄糖转移酶(ecdysteroid UDP-glucosyltransferase，EGT)，使家蚕体内蜕皮激素失去活性，从而延迟家蚕化蛹。其原理是EGT酶可催化将UDP-葡萄糖中的糖基转移至蜕皮激素的反应而使后者失活，从而阻断家蚕的蜕皮和化蛹。本发明解决了鲜茧必须立即烘干杀死蚕茧