SINGLE-STRANDED OLIGONUCLEOTIDE

• Main Authors: IRIYAMA YUSUKE, NIWA MASATOSHI, KANAKI TATSURO, NAKAJIMA HIROYUKI
• Format: Patent
• Language: Chinese; English
• Published: 15.10.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided is a single-stranded oligonucleotide that highly efficiently controls a target gene and can be easily produced. The single-stranded oligonucleotide is expressed by formula (I), wherein X andY hybridize using a first nucleotide sequence section and a second nucleotide sequence section. X has a first nucleotide sequence that consists of 7-100 nucleotides, includes at least one modified nucleotide, and can hybridize with a second oligonucleotide. Y has a second nucleotide sequence that consists of 4-100 nucleotides, allows hybridization with a first oligonucleotide, and includes at least one ribonucleotide. At least one of nucleotide sequences X, Xz, and Y has an antisense sequence that allows hybridization with a target RNA. At least one of L, Lx, and Ly is a linking group that includes a non-nucleotide structure. 本发明提供能高效地控制靶基因、并且能容易地制造的单链寡核苷酸。其为由式(I)表示的、X和Y通过第一核苷酸序列部分和第二核苷酸序列部分进行杂交的单链寡核苷酸。X由7～100个核苷酸形成，且包含至少1个修饰核苷酸，具有能与第二寡核苷酸杂交的第一核苷酸序列。Y由4～100个核苷酸形成，具有能与所述第一寡核苷酸杂交、且包含至少1个核糖核苷酸的第二核苷酸序列。核苷酸序列X、Xz及Y中的至少