Application of phenylpyruvate decarboxylase mutant M538A in biological fermentation production of phenethyl alcohol

• Main Authors: ZHAN YANGYANG, CHEN SHOUWEN, MA XIN, WANG HUAN, XU YONG
• Format: Patent
• Language: Chinese; English
• Published: 15.10.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of gene engineering and enzyme engineering, and discloses an application of a phenylpyruvate decarboxylase mutant M538A in the biological fermentation production of phenethyl alcohol. Through a site-directed mutagenesis way, 538th-site methionine which is from Lactococcus lactis subsp. Lactis of which the preservation number is CICC6246 and is near a phenylpyruvate decarboxylase KivD molecular catalytic structural domain is mutated into alanine. The enzyme activity of the phenylpyruvate decarboxylase is obviously improved, the problem that the existing phenylpyruvate decarboxylase has low catalytic efficiency for phenylpyruvic acid can be solved, the phenylpyruvate decarboxylase mutant M538A is used for the biological fermentation of phenethyl alcohol, a new thought is provided for the production of the phenethyl alcohol, and the phenylpyruvate decarboxylase mutant M538A is suitable for large-scale promotion. 本发明属于基因工程和酶工程技术领域，公开了苯丙酮酸脱羧酶突变体M538A在生物发酵生产苯乙醇中的应用。本发明通过