Preparation method and application of aptamer electrochemical sensor

• Main Authors: WEI LIGANG, ZHANG JINHUA, WANG XIAOMIN, LYU QIAO, YANG YUKUN, GUO CAIXIA, ZHANG GUOHUA, FAN SANHONG
• Format: Patent
• Language: Chinese; English
• Published: 11.10.2019
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a preparation method and application of an aptamer electrochemical sensor. The preparation method comprises the following steps: firstly, activating a screen-printed carbon electrode (SPCE), then dispersing graphene with a solution prepared from poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) and ultrapure water in a volume ratio of 1:1, modifying the surface of the SPCE with graphene dispersion, and drying under an infrared lamp to obtain a graphene-modified SPCE (GR/SPCE); secondly, preparing a nanogold-modified GR/SPCE (AuNPs/GR/SPCE) with an electrodeposition method; and finally modifying the surface of the electrode with an aptamer of which one end is modified with sulfydryl in an Au-S bond covalent binding manner to obtain an aptamer-modified AuNPs/GR/SPCE (Apt/AuNPs/GR/SPCE). According to the method, the surface of a disposable SPCE is modified with the graphene, nanogold and a bisphenol A aptamer to build an aptamer electrochemical sensor amplified on the ba