Somatic cell culturing method for effectively promoting mongolian oak globular embryogeny

• Main Authors: ZHANG LIJIE, ZHANG XIAOLIN, AI WANFENG, ZHAN HAO, HAN XIAOYI, LI MEIYING, LU XIUJUN, MEI MEI, WANG AO, JIN ZIHAN, LI QIAN
• Format: Patent
• Language: Chinese; English
• Published: 17.09.2019
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention provides a somatic cell culturing method for effectively promoting mongolian oak globular embryogeny. The method is characterized by comprising the following steps of selection and treatment of an explant, disinfection for the explant, inoculation, callus induction and somatic embryo induction. The somatic cell culturing method for effectively promoting mongolian oak globular embryogeny has the following advantages that the rate of callus induction and the somatic embryo induction rate are high, and embryonic callus with a small cell volume, compact and thick cytoplasm and a large and clear cell nucleus is obtained; the problems are solved that cutting rooting of mongolian oak is difficult, the propagation coefficient is small, the speed is low, and large-scale production cannot be formed for meeting the market requirements, and by utilizing the cell totipotency, the purpose of large-scale cultivation of good plants in a short period is achieved; the possibility is provided for establishment of