Method for constructing tissue engineered cartilage by using bone-marrow mesenchymal stem cells

The invention provides a method for constructing tissue engineered cartilage by using bone-marrow mesenchymal stem cells, which comprises the steps of (1) preparing a chondrocyte aggregate, (2) centrifuging whole blood to prepare platelet-rich plasma, (3) placing the bone-marrow mesenchymal stem cel...

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Bibliographic Details
Main Authors ZHAO YIMIN, BA RUIKAI, WU WEI
Format Patent
LanguageChinese
English
Published 27.08.2019
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention provides a method for constructing tissue engineered cartilage by using bone-marrow mesenchymal stem cells, which comprises the steps of (1) preparing a chondrocyte aggregate, (2) centrifuging whole blood to prepare platelet-rich plasma, (3) placing the bone-marrow mesenchymal stem cells and the chondrocyte aggregates in the platelet-rich plasma, uniformly mixing, and adding a thrombin solution containing calcium chloride to the obtained mixture to form a cell complex; (4) injecting the cell complex into a subject for growth in vivo to obtain the tissue engineered cartilage, wherein the number ratio of the bone mesenchymal stem cell to chondrocyte contained in the chondrocyte aggregate in step (3) is 1:1. The method for constructing tissue engineered cartilage of the invention is simple in operation, can maintain optimal new cartilage morphology and cartilage differentiation characteristics, and effectively inhibits the occurrence of new cartilage edge ossification whileeffectively resisting cen
Bibliography:Application Number: CN201910434720