一种基于发卡结构变换的放大型荧光生物传感器及制备方法

• Format: Patent
• Language: Chinese
• Published: 11.08.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

本发明提供一种基于发卡结构变换的放大型荧光生物传感器及制备方法，所述传感器包括扩增均相反应液A液、扩增均相反应液B液、发卡DNA序列；所述扩增均相反应液A液，包括：目标miRNA序列、Nt.BstNBI缓冲液、RNA酶抑制剂、dNTP、模板DNA序列；所述扩增均相反应液B液，包括：去离子水、切口核酸内切酶Nt.BstNBI缓冲液、Vent（exo）DNA聚合酶、ThermoPol缓冲液。本发明扩增出的大量靶标适体与发卡结合，仅通过发卡的结构反转，即可实现对信号的放大与衰减。操作简单、快捷。 The invention provides an amplified fluorescence biosensor based on hairpin structure transformation and a preparation method thereof. The biosensor comprises an amplification homogeneous reaction solution A, an amplification homogeneous reaction solution B and a hairpin DNA sequence. The amplification homogeneous reaction solution A comprises a target miRNA sequence, a Nt.BstNBI buffer solution,an RNA enzyme inhibitor, dNTP and a template DNA sequence. The amplification homogeneous reaction solution B comprises deionized water, an incision endonuclease Nt.BstNBI buffer solution, Vent(exo-)DNA polymerase and a ThermoPol buffer solution. A large quantity of target aptamers and hairpins are amplified, and signal amplification and attenuation can be achieved just throu