Method for evaluating sperm viability of bulls based on FBXW11 methylation and application

• Main Authors: WANG LINGLING, JIANG QIANG, WANG XIUGE, WANG JINPENG, LI YANQIN, GAO YAPING, YANG CHUNHONG, WEI XIAOCHAO, LIU WENHAO, ZHAO HAN, HUANG JINMING, ZHANG YARAN, LIU YONG, JU ZHIHUA, SUN YAN
• Format: Patent
• Language: Chinese; English
• Published: 20.08.2019
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TESTING; TRAPPING; VINEGAR; WINE

The invention belongs to the technical field of livestock molecule heredity biology, and particularly relates to a method for evaluating sperm viability of bulls based on FBXW11 methylation and an application. High-flux sequencing is performed on a bull semen transcriptome. A FBXW11 gene and 11 methylation sites influencing the sperm viability of the bulls are provided, through functional annotation and data confluence analysis, a methylation site mark for evaluating the degree of the sperm viability of the bulls is developed, and a Bisulfite sequencing PCR detection method for several key methylation sites is established and can be used for evaluating the sperm viability of breeding bulls. Through the adoption of the technical scheme of the invention, the DNA methylation difference situation of the gene is analyzed, so that individuals or semen samples carrying ideal properties are screened, bulls having high fecundity and semen having high sperm viability are bred out, the propagation efficiency of the bree