Microbial flocculant preparation method

• Main Authors: JIANG BINHUI, ZHANG LUNQIU, ZHANG YAN, FU LILI, WEI JIANWEI, LIU JINLIANG, TONG SHUYANG, TIAN WEI, LIANG JILING, WANG WEIQIANG
• Format: Patent
• Language: Chinese; English
• Published: 16.08.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE; VINEGAR; WINE

The invention relates to the technical field of microorganisms and discloses a microbial flocculant preparation method. By setting of different culture time, in culture time of 0-22h, culture flocculation activity is improved along with culture time increase, the flocculation rate is maximum in culture of 66h and starts to decrease after 66h, and thus, a user can obtain most suitable time for culture flocculation activity, and product quality is effectively improved. By comparison of an acetone extraction method, an ethanol extraction method and a hexadecyl trimethyl ammonium bromide extraction method, the hexadecyl trimethyl ammonium bromide extraction method is high in yield, 1.33g of a dry product can be obtained by each liter of culture solution, the flocculation rate in the hexadecyltrimethyl ammonium bromide extraction method is high relatively and reaches 71.8%, activity of flocculant crude products is effectively improved, and a flocculant extracted from microbial culture solution according to the hex