Preparation method of biological 3D printing ink by adjusting gel-sol phase-transition temperature

• Main Authors: SHI TINGCHUN, YAO DANYU, LI MEIQI
• Format: Patent
• Language: Chinese; English
• Published: 16.08.2019
• Subjects: AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS OF MACROMOLECULAR COMPOUNDS; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; GENERAL PROCESSES OF COMPOUNDING; HUMAN NECESSITIES; HYGIENE; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEDICAL OR VETERINARY SCIENCE; METALLURGY; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; WORKING-UP

The invention discloses a preparation method of a biological 3D printing ink by adjusting gel-sol phase-transition temperature. The method controls the sol-gel phase-transition temperature of a gelatin/sodium alginate 3D printing hydrogel system to control the temperature in a most appropriate temperature range for cell growth, and a cellulosic 3D printing technology is then used to print a high-resolution cartilage scaffold to improve the printability and biocompatibility of the hydrogel material. The inorganic nanoparticles are added to the basic hydrogel system can improve the mechanical properties of the cartilage scaffold, which can solve the technical problem that the printing moulding temperature is not matched with the cell growth temperature and the mechanical properties of the hydrogel material are not good. 本发明公开了一种可调控凝胶-溶胶相变温度的生物3D打印墨水制备方法，本发明通过调控明胶/海藻酸钠3D打印水凝胶体系的溶胶-凝胶相变温度，将之控制在最适合细胞生长的温度范围内。然后利用细胞3D打印技术打印出分辨率高的软骨支架，提高水凝胶材料的可打印性和生物相容性。将无机纳米颗粒加入到此基础水凝胶体系中，可以提高软骨支架的力学性能，从而解决打印成型温度与细胞生长温度不匹配和水凝胶材料力学性