RECOMBINANT YEAST STRAINS FOR PENTOSE FERMENTATION

• Main Authors: SANCHEZ I NOGUE VIOLETA, KARHUMAA KAISA, SENDELIUS MALIN
• Format: Patent
• Language: Chinese; English
• Published: 09.08.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Described herein are recombinant yeast cells expressing a xylulose kinase (XK) whihch are suitable for fermentation of pentoses. Also described are recombinant yeast cells with higher toleranance to formic and/or acetic acid and suitable for fermentation of pentoses. Also described are recombinant yeast cells expressing an enolase, a phophofructokinase beta subunit, a 6-phosphofructo-2-kinase, a glucose-6-phosphate isomerase, a phosphoglycerate mutase and/or a triose-phosphate isomerase, and suitable for fermentation of pentoses. Also described are recombinant yeast cells expressing a a phosphoglucomutase and/or phosphoribomutase which are suitable for fermentation of pentoses. Further described are are methods of using or producing such recombinant yeast cells and related materials. 本文描述了表达木酮糖激酶(XK)的重组酵母细胞，该重组酵母细胞适用于戊糖发酵。还描述了对甲酸和/或乙酸具有更高的耐受性并且适用于戊糖发酵的重组酵母细胞。还描述了表达烯醇酶、磷酸果糖激酶β亚基、6-磷酸果糖-2-激酶、葡萄糖-6-磷酸异构酶、磷酸甘油酸变位酶和/或丙糖磷酸异构酶，并且适合于戊糖发酵的重组酵母细胞。还描述了表达磷酸葡萄糖变位酶和/或磷酸核糖变位酶的重组酵母细胞，该重组酵母细胞适用于戊糖发酵。进一步描述了使用或生产此类重组酵母细胞的方法和相关材