POL6 POLYMERASE VARIANTS

• Main Authors: WOERSDOERFER BIGNA, BOENITZ-DULAT MARA, SARVABHOWMAN PREETHI, SCHWAB CHARLES WAYAN, LEDERMAN ILYA, AMATO EVAN, CLEOMA ARNOLD, AYER ARUNA, WUNDERLICH DAVID, ECKERT BARBARA, SUKO SHAWN, THAI EILEEN, MCGAW COLIN
• Format: Patent
• Language: Chinese; English
• Published: 09.08.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nano-pore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived. 本公开内容提供了变体Pol6聚合酶多肽，包含Pol6变体多肽的组合物，以及使用所述变体Pol6多肽来测定核酸测序（例如，通过纳米孔测序）的方法。变体Pol6聚合酶具有降低的模板与聚合酶-模板复合物的解离速率，这导致相对于衍生它们的亲本Pol6多肽增加的持续合成能力。