Kit for diagnosing IgA nephropathy (IgAN) and application of kit

• Main Authors: LYU GUANGXIN, ZHENG FENG, YE MINGLIANG, SHENG JIE, QIN HONGQIANG
• Format: Patent
• Language: Chinese; English
• Published: 09.08.2019
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a kit for diagnosing IgA nephropathy (IgAN). The kit comprises a reagent capable of capturing IgA1 in an in-vitro sample, wherein the reagent is TINAGL1 protein, plasmid capableof stably expressing the TINAGL1 protein or a cell capable of expressing the TINAGL1 protein obtained by transfection of the plasmid capable of stably expressing the TINAGL1 protein; and an immunofluorescence method can be utilized, the IgA1 in the in-vitro sample is detected through the formation of an IgA1-TINAGL1 immune complex to diagnose the occurrence of the IgAN, and non-invasive detectionof the IgA nephropathy is realized. Meanwhile, the invention provides a new biomarker TINAGL1 capable of forming a complex with IgA1 in circulatory tissues such as blood of IgAN patients and capableof being used for diagnosing the IgAN, a new direction is provided for treatment and research of the IgAN, and application value is wide. 本发明提供一种用于诊断IgA肾病的试剂盒，该试剂盒包括能够捕捉离体样品中的IgA1的试剂，该试剂为TINAGL1蛋白、能够稳定表达TINAGL1蛋白的质粒或将所述能够稳定表达TI