Method for hydroponic proliferation of tissue culture seedlings of thesium chinense Turcz

• Main Authors: TAN XIANRUI, XIANG ZENGXU, ZHANG CHENGCAI
• Format: Patent
• Language: Chinese; English
• Published: 09.08.2019
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED; FISHING; FORESTRY; HORTICULTURE; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING; WATERING

The invention relates to a method for hydroponic proliferation of tissue culture seedlings of thesium chinense Turcz.. The method comprises the steps of firstly, taking strong terminal buds of thesiumchinense Turcz., cleaning and disinfecting the terminal buds, and then inoculating the terminal buds into an MS culture medium for culture; secondly, transferring the terminal buds into a cluster budinduction culture medium for culture, inoculating generated adventitious buds into a proliferation culture medium for seedling strengthening proliferation culture, and obtaining the tissue culture seedlings of thesium chinense Turcz.; selecting the strong tissue culture seedlings dark green in color, transferring the tissue culture seedlings into an artificial climate chamber, opening a cover forhardening of the seedlings to obtain hydroponic mother seedlings of thesium chinense Turcz., planting the hydroponic mother seedlings into a hydroponic tank filled with a proliferation nutrient solution, and performing hydropo