COMPOSITIONS AND METHODS FOR ENHANCING GROWTH, SPREAD, AND ONCOLYTIC AND IMMUNOTHERAPEUTIC EFFICACY OF ONCOLYTIC RNA VIRUSES

• Main Authors: DIALLO JEAN-SIMON, SELMAN MOHAMMED
• Format: Patent
• Language: Chinese; English
• Published: 16.07.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

Provided herein are methods for enhancing infection, growth, spread, or titer of an oncolytic RNA virus in a cancer or tumor cell; enhancing the oncolytic activity, cytokine-induced cell death activity, and/or cytotoxic activity of an oncolytic RNA virus in a cancer or tumor cell; upregulating cytokine response to, and/or enhancing the immunotherapeutic activity of an oncolytic RNA virus in a cancer or tumor cell; and/or treating a tumor or cancer in a subject in need thereof. Such methods employ a vanadium-containing compound, administered to the cancer or tumor cells before, after, or concurrently with infection of the cancer or tumor cells with the oncolytic RNA virus. Related compositions, uses, and kits therefor are also provided. Methods for producing RNA viruses, RNA virus-based cancer vaccines, and RNA virus-based cancer gene therapy vectors are also provided. 本文中提供了用于在癌症或肿瘤细胞中提高溶瘤RNA病毒的感染、生长、传播或滴度，在癌症或肿瘤细胞中提高溶瘤RNA病毒的溶瘤活性、细胞因子诱导的细胞死亡活性和/或细胞毒活性，在癌症或肿瘤细胞中上调对溶瘤RNA病毒的细胞因子应答和/或提高溶瘤RNA病毒的免疫治疗活性，和/或在需要的对象中治疗