CLOSED LINEAR DNA PRODUCTION

• Main Authors: ROTHWELL PAUL, PORTER NEIL, ADIE THOMAS
• Format: Patent
• Language: Chinese; English
• Published: 04.06.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to improved processes for production of closed linear deoxyribonucleic acid (DNA), in particular cell-free enzymatic production of closed linear DNA molecules, preferablyusing a closed linear DNA as a template for DNA synthesis. The invention further relates to a novel closed linear DNA species, suitable for use as a template in the improved processes for productionof closed linear DNA. Further, the invention pertains to the intermediate products of the processes, since this enables the production of larger quantities of closed linear DNA from the template thanwith methods known in the art. 本发明涉及用于生产闭合线性脱氧核糖核酸(DNA)的改良方法，尤其是用于在无细胞下酶促生产闭合线性DNA分子，优选使用闭合线性DNA作为DNA合成的模板。本发明还涉及新型的闭合线性DNA物质，其适合作为模板用在生产闭合线性DNA的改良方法中。另外，本发明还涉及所述方法的中间产物，原因是这能够使由模板生产的闭合线性DNA的数量大于现有技术已知的方法。