CELL THERAPY WITH POLARIZED MACROPHAGES FOR TISSUE REGENERATION

• Main Authors: SOLA MARTINEZ ANA MARIA, CASTILLO GARCIA ADRIAN, RODRIGUEZ GARCIA ANNA, HOTTER CORRIPIO MARIA GEORGINA, DOMINGUEZ SANCHEZ RUBEN, GINESTA BUCH XAVIER, MARTIN CORDERO JORGE VICENTE, SANCHEZ MORENO JAIME, GARCIA DE LA RIVA MESTRE PABLO
• Format: Patent
• Language: Chinese; English
• Published: 04.06.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides an in vitro method for inducing macrophage polarization to an M2 phenotype useful for tissue repair. The method described in the present invention comprises the in vitro exposure of macrophages to repeated series of hypoxia- reoxygenation. Activated M2 macrophages obtained by this method overexpress molecules important for tissue remodeling and amelioration of inflammation,such as NGAL and anti-inflammatory cytokines (IL-10). Thus, M2 macrophages obtained by this method are useful as cell therapy for tissue regeneration. The invention also provides pharmaceutical compositions and kits comprising the M2 macrophages obtained by the described method. The invention further refers to a device for inducing hypoxia and re- oxygenation conditions on isolated macrophages according to the described method. 本发明提供了诱导巨噬细胞极化为可用于组织修复的M2表型的体外方法。本发明所述方法包括将巨噬细胞体外暴露于重复系列的缺氧-复氧。通过该方法获得的活化的M2巨噬细胞过表达对组织重塑和炎症改善重要的分子，例如NGAL和抗炎细胞因子(IL-10)。因此，通过该方法获得的M2巨噬细胞可用作用于组织再生的细胞疗法。本发明还提供包含通过所述方法获得的M2巨噬细胞的药物组合物和试剂盒。本发明还涉及根