Double-enzyme processing method for glycoprotein

The application relates to a double-enzyme processing method for glycoprotein. The method is characterized in that glycosidase and trypsin are simultaneously added into a sample to carry out processing. The double-enzyme processing method disclosed by the application can be used for processing the g...

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Bibliographic Details
Main Authors LIANG CHENGGANG, WANG LYUYIN, ZHANG HUI, LI JING, SUN YUE, LI ZHANJUN, ZHANG WEI, LYU PING, LI YI
Format Patent
LanguageChinese
English
Published 07.05.2019
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:The application relates to a double-enzyme processing method for glycoprotein. The method is characterized in that glycosidase and trypsin are simultaneously added into a sample to carry out processing. The double-enzyme processing method disclosed by the application can be used for processing the glycoprotein such as rhFSH (recombinant human Follicle Stimulating Hormone), and for example, used for N-end non-uniformity analysis and peptide map detection of the rhFSH. 本申请涉及糖蛋白的双酶处理法，是将糖苷酶与胰蛋白酶同时加入样品中进行处理。本申请的双酶处理法可用于处理诸如重组人促卵泡激素(rhFSH)这样的糖蛋白，例如用于rhFSH的N端不均一性分析和肽图检测。
Bibliography:Application Number: CN201910042888