Sporosarcina psychrophila and application thereof

• Main Authors: KANG YAXIN, FENG LU, LI DONGPENG, ZHAO XIAOXIANG, LIU JINGYU, KANG XIN
• Format: Patent
• Language: Chinese; English
• Published: 03.05.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE; VINEGAR; WINE

The present invention relates to a sporosarcina psychrophila and an application thereof. The sporosarcina psychrophila KX-1 has a preservation number of CGMCC No. 16770, has a nucleotide sequence as shown in SEQ ID NO.1, and is used for degrading 3-chlorocarbazole. The most suitable degradation conditions for the strain of the present invention are as follows: 3-chlorocarbazole concentration is 50mg/L, the inoculum amount is 10%, the pH value is 6.0, the temperature is 30 DEG C, and an additional 1 g/L soluble starch solution is a supplementary carbon source. Under the condition, after 5 days, the degradation rate of the 3-chlorocarbazole by the sporosarcina psychrophila reaches 90.0%. 本发明涉及一种嗜冷芽孢八叠球菌及其应用，为嗜冷芽孢八叠球菌(Sporosarcina psychrophila)KX-1，保藏号为CGMCC No.16770，核苷酸序列如SEQ ID NO.1所示。用于降解3-氯咔唑。本发明菌株最适宜的降解条件为：3-氯咔唑浓度为50mg/L，接种量为10％，pH为6.0，温度为30℃，外加1g/L可溶淀粉溶液为补充碳源，在此条件下，5d后，嗜冷芽孢八叠球菌对3-氯咔唑的降解率达到90.0％。