Method for identifying bacteria

• Main Authors: GE LIANGHAO, WANG YIQIAN, XU JIAN, GOU HONGLEI
• Format: Patent
• Language: Chinese; English
• Published: 30.04.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention provides a method for identifying bacteria or identifying drug-resistant bacteria. With a surface-enhanced Raman scattering technique based on a noble metal nanoparticle active substrate, Raman detection is carried out on the specific stress secretion by combining the stress reaction mechanism to the external pressure by the bacteria, so that the bacteria type or the drug-resistant performance of the bacteria can be distinguished and determined rapidly. 本发明提供了一种细菌鉴别或耐药细菌鉴别的方法，采用基于贵金属纳米颗粒活性基底的表面增强拉曼散射技术，结合细菌对外源压力的应激反应机制，对其特异性的应激分泌物进行拉曼检测，实现了对细菌类别或是否具有耐药性进行快速区分和判断。