Primers and kit for detecting avian influenza virus H5, H7 and H9 subtype triple RT-PCR

• Main Authors: ZOU WEIHUA, DAN HANBING, JIA HUIQIN, DONG XIAOHUI, ZHOU YUNDUO, FAN JUNQING, LI PANPAN, XU GAOYUAN, MA LEI, ZHANG CHI
• Format: Patent
• Language: Chinese; English
• Published: 16.04.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses primers for detecting avian influenza viruses H5, H7 and H9 subtype triple RT-PCR, which comprise three pairs of specific primers, namely, a first primer pair: H5-F and H5-R, asecond primer pair: H7-F and H7-R, and a third primer pair: H9-F and H9-R, wherein the primers respectively have base group sequences of sequence tables SEQ ID NO. 1 to SEQ ID NO. 6. Aiming at HA genes of three subtypes of avian influenza viruses, H5, H7 and H9, which are popular at present, the three pairs of specific primers are designed through sequence comparison to form an avian influenza virus H5, H7 and H9 subtype triple RT-PCR detection kit, meanwhile, reverse transcription and PCR amplification one-step reaction are adopted, after the amplification is finished, detection results areintuitively obtained through agarose gel electrophoresis, and the three subtypes of pathogens of H5, H7 and H9 can be simply, conveniently and quickly detected in one tube, so that avian influenza subtype identification is car