CircRNA related to MTX resistance of tumor cells and application of circRNA to reversal of MTX resistance of tumor cells

• Main Authors: MENG XIANGNING, WANG PING, GAO WEI, CAI MENGDI, ZHANG HUISHU, LYU YAFAN, ZHU JING, WANG XU, GUAN RONGWEI, HAN YUE, MA JINFA, SONG YING
• Format: Patent
• Language: Chinese; English
• Published: 09.04.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a circRNA related to MTX resistance of tumor cells and application of the circRNA to reversal of the MTX resistance of the tumor cells. An HT-29 MTX-sensitive and DMs-containing MTX-resistant cell line is selected as a study subject, the circRNA highly expressed in MTX-resistant cells is obtained through second-generation sequencing, validation and optimization and is namedas circRNA7746, and the expression quantity of the circRNA7746 is further determined to be positively correlated to the degree of the methotrexate (MTX) resistance of the tumor cells through establishment of an MTX treatment model of a tumor-bearing mouse and in combination with PCR and an agarose gel electrophoresis method. According to a virus transfection method, influence of the interfering circRNA7746 on the drug resistance of the cells, the amplification degree and the amplification form of a DHFR gene and micronuclear excretion is detected respectively, and the facts that inhibition ofthe circRNA7746 can promo