MEASUREMENT OF Lp-PLA2 ACTIVITY

• Main Authors: SUGIMORI DAISUKE, YAMAURA SAKI, SAKASEGAWA SHINICHI
• Format: Patent
• Language: Chinese; English
• Published: 02.04.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

A purpose of the present invention is to provide a highly versatile, simple and safe method for measuring Lp-PLA2 activity. Another purpose of the present invention is to provide an accurate and highly sensitive method for measuring Lp-PLA2 activity. Provided is a method for measuring Lp-PLA2 activity in a sample containing Lp-PLA2, said method comprising steps (A) to (C): (A) a step for reactingPAFs with Lp-PLA2 in the sample and thus converting the PAFs into lyso-PAFs; (B) a step for hydrolyzing the lyso-PAFs formed in step (A) with an enzyme (lyso-PAF-PLD ) to give hydrolysates; and (C) astep for measuring the Lp-PLA2 activity in the sample with the use of a quantitative change derived from the hydrolysates obtained in step (B) as an indication. 本发明的目的在于提供种通用性高、简便、安全的Lp-PLA活性的测定方法。另外，目的在于提供种准确、高灵敏度的Lp-PLA活性的测定方法。本发明提供种包含Lp-PLA的试样中的Lp-PLA活性的测定方法，该测定方法包括以下的工序(A)～工序(C)：(A)使PAF类与试样中的Lp-PLA反应而转换为Lyso PAF类的工序；(B)利用酶(Lyso PAF-PLD)将工序(A)中生成的上述Lyso PAF类水解而得到水解物的工序；(C)对于工序(B)中得到的水解物，以来自该水解物的变化量为指标，对试样中的Lp-PLA活性进行测定的