Fluorescent in-situ hybridization sequencing method for detecting single nucleotide polymorphisms of NR1I2A gene

• Main Authors: WANG HEYAO, SUN MEINA
• Format: Patent
• Language: Chinese; English
• Published: 22.03.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a fluorescent in-situ hybridization sequencing method for detecting single nucleotide polymorphisms of a NR1I2A gene. The method comprises the following steps: extracting the DNA (Deoxyribonucleic Acid) of a sample to be detected, and performing single-chain derivation by taking the DNA as a template; then adding a first sequencing probe and a second sequencing probe with different fluorescent labels simultaneously for hybridizing with a single-chain derivative; and finally interpreting a hybridization result. The method has the advantages of high accuracy, good stability, rapidness, safety, and easiness in automatic operation. By adopting the method, accurate typing of the single nucleotide polymorphisms can be finished in one single-chain derivation and hybridization reaction cycle, so that the genotype of a subject can be known, and prevention of diseases caused by dangerous factors and guidance for clinical individualized administration are realized. 本发明公开了种检测NR1I2A基因单核苷酸多态性的荧光原位杂交