Carbonyl reductase EbSDR8 mutant as well as construction method and application thereof

• Main Authors: MA ZONGJIE, NIU SHANPO, YU XINYAN, ZHANG JINGPENG
• Format: Patent
• Language: Chinese; English
• Published: 15.03.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a carbonyl reductase EbSDR8 mutant as well as a construction method and application thereof, wherein the amino acid sequence of carbonyl reductase EbSDR8, as shown in SEQ ID NO.2, has single-point mutation or two-point combination mutation in 97th-site alanine or 160th-site lysine, wherein the 97th-site alanine is mutated to leucine and the amino acid sequence of the leucine is as shown in SEQ ID NO.4; the 160th-site lysine is mutated to glutamic acid and the amino acid sequence of the glutamic acid is as shown in SEQ ID NO.6. The invention aims at providing the carbonyl reductase EbSDR8 mutant as well as the construction method and application thereof, and the catalytic activity of the carbonyl reductase EbSDR8 is improved. 本发明公开了种羰基还原酶EbSDR8突变体及其构建方法和应用。其中，羰基还原酶EbSDR8的如SEQ ID NO.2所示的氨基酸序列中具有97位丙氨酸或160位赖氨酸中的单点突变或两点组合突变，其中，97位丙氨酸突变为亮氨酸，具有如SEQ ID NO.4所示氨基酸序列；160位赖氨酸突变为谷氨酸，具有如SEQ ID NO.6所示氨基酸序列。本发明的目的在于提供种羰基还原酶EbSDR8突变体及其构建方法和应用，提高羰基还原酶EbSDR8的催化活性。