ENGINEERED MICROBES AND METHODS FOR MICROBIAL OIL PRODUCTION

• Main Authors: SAGAR CHAKRABORTY, GREGORY STEPHANOPOULOS, MITCHELL TAI
• Format: Patent
• Language: Chinese; English
• Published: 15.03.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to engineered microbes and methods for microbial oil production. Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties. 本发明涉及工程改造的微生物及用于微生物油生产的方法。本发明的些方面提供了用于油生产的工程改造的微生物。本文还提供了用于对微生物进行工程改造的方法及工程改造的微生物的用途。在些实施方式中，提供了微生物，其经过工程改造而调制了脂质合成的速率控制步骤的组合，例如生成代谢物乙酰-CoA、ATP或NADPH用于脂质合成的步骤(推步骤)和隔离脂质合成途径中介导脂质合成的反馈抑制的产物或中间产物的步骤(拉步骤)的组合。这种推-和-拉工程改造的微生物显示了极大增强的转化产率、TAG