Molecular marking method for identifying gramineous pasture

• Main Authors: HAN WEIBO, WANG JIANLI, SHEN ZHONGBAO, ZHONG PENG, DI GUILI, MA LICHAO, LIU JIELIN, ZHU RUIFEN
• Format: Patent
• Language: Chinese; English
• Published: 08.03.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a specific molecular marker primer for identifying gramineous pasture and a molecular marking method for identifying gramineous pasture. The method comprises the specific steps:separately extracting DNAs of different gramineous pasture to be detected; preparing an amplifying reaction system and carrying out PCR amplification; carrying out agarose gel electrophoresis detection on a PCR product; sequencing the PCR product. The method can identify different gramineous pasture within a short time by means of molecular markers. The method is suitable for identifying a plurality of gramineous pasture varieties, has the advantages of being simple and feasible, convenient to operate, quick to take effect and the like, and provides beneficial reference to phylogenetic analysis of different gramineous pasture. 本发明提供了种鉴别禾本科牧草的特异性分子标记引物对以及鉴别禾本科牧草的分子标记方法，具体包括，分别提取待检测的不同禾本科牧草的DNA；配制扩增反应体系并进行PCR扩增；PCR产物进行琼脂糖凝胶电泳检测；对PCR产物测序。本发明通过分子标记短时间内鉴别不同禾本科牧草，该方法适用于多个禾本科牧草品种的鉴定，具有简单易行，操作便捷，见效快等优点，为不同禾本科牧草的系统发育分析提供有