Method and SSR primers for purity identification of melon hybrid Huangjinmi 25

• Main Authors: LU XIA, DENG ZHIJUN, LIN AIHUA, FAN WENYI, SI LONGTING, TENG LUHUA, A MEN
• Format: Patent
• Language: Chinese; English
• Published: 08.03.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses SSR primers for purity identification of melon hybrid Huangjinmi 25. The SSR primers include a primer pair gSSR15606 and/or a primer pair Gssr45335 and can generate specific markers in Huangjinmi 25 parents, and high specificity is realized. The invention further provides a method for purity identification of melon hybrid Huangjinmi 25. According to the method, melon hybridseed purity can be identified quickly, accurately and efficiently by the SSR primers. By the primer and the method, purity of the melon hybrid seeds can be effectively identified only through simpleextraction of melon leaf genome DNA, PCR amplification and electrophoresis. Hybrid seed purity identification can be completed within 5h according to the method, and the method has advantages of quickness, accuracy, low cost, convenience in operation and the like, can be used for substituting a traditional hybrid seed purity identification method and is high in commercial application value. 本发明公开了种用于甜瓜杂交种黄金蜜25纯度鉴定的SSR引物，所述