Biological surfactant bacterial strain production CTAB (cetyltrimethyl ammonium bromide) screening method

• Main Authors: QI YIBIN, MA TAO, WANG HAITAO, LYU CHENGYUAN, LUN ZENGMIN
• Format: Patent
• Language: Chinese; English
• Published: 05.03.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of petroleum microorganism and particularly relates to a biological surfactant bacterial strain production CTAB screening method. The biological surfactantbacterial strain production CTAB screening method comprises the following steps of (1) sampling including selecting bacteria-containing water samples; (2) enrichment culture including mixing biological surfactant production activator with the bacteria-containing water samples in a sealed mode, performing shake culture to obtain an enriched bacteria solution; (3) CTAB plate separation including diluting the enriched bacteria solution, applying the diluted enriched bacteria solution onto a CTAB-containing plate for culture until dark blue halo-like bacterial colonies are formed onto the CTAB-containing plate, and inoculating single colonies inside the dark blue halo of the CTAB plate into a culture medium for shake culture; (4) strain secondary screening and capability measurement includingscreening out alternative