EXTRACTION OF CIRCULATING TUMOR CELLS

• Main Author: JOSEFSSON ANDREAS
• Format: Patent
• Language: Chinese; English
• Published: 01.03.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present disclosure relates to a method of extracting circulating tumor cells (CTCs) from a blood sample from a human subject. This method includes adding a hyaluronan degrading enzyme to a blood sample from a subject in order to remove hyaluronan from the surface of the CTCs, bringing a CTC-capturing agent in contact with the CTCs and separating captured CTCs from the blood sample. 本公开内容涉及种从来自人类受试者的血液样品中提取循环肿瘤细胞(CTC)的方法。此方法包括将透明质酸降解酶添加至来自受试者的血液样品中以便从CTC的表面去除透明质酸，使CTC-捕获剂与CTC接触并且将被捕获的CTC与血液样品分离。