Immune-enhanced recombinant PRRSV (Porcine Reproductive and Respiratory Syndrome Virus)-like particle subunit vaccine

• Main Authors: DONG JIANHUI, CHEN RUI, DU ENQI
• Format: Patent
• Language: Chinese; English
• Published: 01.03.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses an immune-enhanced recombinant PRRSV (Porcine Reproductive and Respiratory Syndrome Virus)-like particle subunit vaccine. The immune-enhanced recombinant PRRSV virus-like particle subunit vaccine consists of PRRSV-like particles and a compound immunologic adjuvant. The immune-enhanced recombinant PRRSV-like particle subunit vaccine disclosed by the invention has the beneficial effects that by the means of gene engineering, PRRSV GP5-M gene is modified, and the PRRSV-like particles with high immunogenicity are prepared by constructing rhabdovirus expression vectors. In addition, by improvement on the immunologic adjuvant, the immune-enhanced recombinant PRRSV-like particle subunit vaccine is obtained and the subunit vaccine has a better immune effect. 本发明公开了种免疫增强型重组PRRSV病毒样颗粒亚单位疫苗，由PRRSV病毒样颗粒和复合免疫佐剂组成。本发明通过基因工程的手段，改造了PRRSV GP5-M基因，通过构建杆状病毒表达载体制备得到具有高免疫原性的PRRSV病毒样颗粒。另外，本发明通过免疫佐剂的改进，得到了种免疫增强型重组PRRSV病毒样颗粒亚单位疫苗，该亚单位疫苗具有更好的免疫效果。