Detection method for components of raw material and preparation of Rifaximin

• Main Authors: QIN FENG, QIAN CHEN, LIU HAO, ZHAO JINGDAN
• Format: Patent
• Language: Chinese; English
• Published: 19.02.2019
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a detection method for components of the raw material and the preparation of the Rifaximin, which is a supercritical fluid chromatography. The method comprises the following steps of: dissolving the raw material or the preparation of the Rifaximin by an organic solvent; carrying out gradient elution by using CO2 and lower alcohols as mobile phase; and determining the components and the content thereof by adopting a detector, wherein the detected components comprise the Rifaximin and impurities including any one or more of 2-amino-4-methylpyridine, rifamycin B, rifamycin SV, rifaximin Y, rifamycin S, rifamycin O, impurity A, impurity B, hydroxyrifaximin. The detection method provided by the invention is good in specificity, high in analysis speed, good in repeatability, the degree of separation of Rifaximin and impurities is good, can be used directly with a mass spectrometer, convenient in structure speculation of impurities, efficient data support can be provided for evaluating the q