Simple preparation method of DNA-Maker

• Main Authors: SUN TONGCHUAN, DAI FA, LI LIMEI, BU LIANGHAO, CHEN KAIZHAO, OUYANG LEJUN, GUO JUN, HUANG JIALING, MA MINGSAI
• Format: Patent
• Language: Chinese; English
• Published: 19.02.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a simple preparation method of a DNA-maker, comprising the steps of subjecting plasmids p12S-MK and p12F-MK to enzyme digestion via EcoR I restriction enzyme, purifying the digestion product, recycling the purified product, adding a storing liquid and a loading buffer, and mixing. The simple preparation method herein has high stability; repeated production between lots canbe ensured; large-scale production can be achieved; the production process is simple; after a carrier is constructed in the early stage, the production cost of DNA-maker is very low in later stage; the prepared DNA-maker has clear stripes evenly distributed, has stable quality and is suitable for long-term storage. In addition, the enzyme digestion system and time are optimized, enzyme digestion efficiency is greatly improved, and preparation time is shortened. 本发明公开了种DNA-Maker的简易制备方法，其步骤为：将p12S-MK、p12F-MK两种质粒用EcoR Ⅰ限制性内切酶进行酶切，对酶切产物进行纯化回收后，加储存液、Loading buffer混合即可。本方法稳定性高，可以确保批次之间的重复性生产，从而实现大规模生产，生产过程简单，前期载体构建好之后，后期DNA