Syngonium podophyllum golden regeneration system building method

• Main Authors: DAI FA, YANG JIAFENG, LI LIMEI, BU LIANGHAO, CHEN KAIZHAO, ZHANG YANSHAN, OUYANG LEJUN, HU JIANWEN, HUANG JIALING
• Format: Patent
• Language: Chinese; English
• Published: 19.02.2019
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention belongs to the technical field of plant regeneration by tissue culture techniques, and discloses a syngonium podophyllum golden regeneration system building method. An elongation mediumMS+7g/L agar+30g/L sucrose+100mg/L Vc+0.2mg/L IBA (indolebutyric acid)+0.5mg/L IAA (indoleacetic acid)+0.4mg/L PBU (phenyl butyl urea) is added into an MS medium. A rooting medium is MS+7g/L agar+30g/L sucrose+100mg/L Vc+0.5mg/L IBA, and a bud multiplication medium is MS+7g/L agar+30g/L sucrose+100mg/L Vc+1.0mg/L NAA (nicotinic acid amide)+1.0mg/L PBU. The multiplication coefficient and the rooting rate of syngonium podophyllum golden adventitious buds are increased, and elongation of the adventitious buds is promoted. 本发明属于通过组织培养技术的植物再生技术领域，公开了种金童子合果芋再生体系的建立方法，在MS培养基中添加伸长培养基：MS+7g/L琼脂+30g/L蔗糖+100mg/L Vc+0.2mg/L IBA+0.5mg/L IAA+0.4mg/L PBU；生根的培养基为：MS+7g/L琼脂+30g/L蔗糖+100mg/L Vc+0.5mg/L IBA；芽增殖的培养基为MS+7g/L琼脂+30g/L蔗糖+100mg/L Vc+1.0mg/L NAA+1.0mg/LPBU。本发明提高了金童子合果芋不定芽的增殖系数、生根率，促进了不定芽的伸长。