Determination method of content of astragaloside IV in Zuo Huang Tong Mi soft-capsule

• Main Authors: ZHOU YONGYAN, QU YUNPING, LI FEI, ZHOU YANAN, JIANG GUOZHI, SUN SHENGBIN
• Format: Patent
• Language: Chinese; English
• Published: 15.02.2019
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a determination method of the content of astragaloside IV in a Zuo Huang Tong Mi soft-capsule. A processing method of a solution of a sample for testing comprises: weighing andtaking contents of the soft capsule, adding diatomite for even grinding, adding a concentrated ammonia methanol solution, and carrying out heating refluxing or ultrasonic extraction to obtain an extract; filtering the extract, precisely measuring and taking a filtrate for evaporating to dryness, adding water to residues to enable the same to be dissolved, and then using water-saturated n-butanolfor extraction to obtain an extract; concentrating the extract to dryness, and adding methanol for capacity determination to obtain the solution of the sample for testing. The determination method ofthe invention has higher specificity and good reproducibility, a negative control has no interference, a baseline of an obtained chromatogram is stable, peak shapes of chromatographic peaks are good,baseline separation is achie