METHODS AND COMPOSITIONS FOR THE MAKING AND USING OF GUIDE NUCLEIC ACIDS

• Main Author: GOURGUECHON STEPHANE B
• Format: Patent
• Language: Chinese; English
• Published: 05.02.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided herein are methods and compositions to make guide nucleic acids (gNAs), nucleic acids encoding gNAs, collections of gNAs, and nucleic acids encoding for a collection of gNAs from any source nucleic acid. Also provided herein are methods and compositions to use the resulting gNAs, nucleic acids encoding gNAs, collections of gNAs, and nucleic acids encoding for a collection of gNAs in a variety of applications. 本文提供了从任何来源的核酸制备指导核酸(gNA)、编码gNA的核酸、gNA的集合和编码gNA的集合的核酸的方法和组合物。本文还提供了在多种应用中使用所得的gNA、编码gNA的核酸、gNA的集合和编码gNA的集合的核酸的方法和组合物。