Method for detecting different beta-casein variants in milk

• Main Authors: WANG LINGLING, WEI XIAOCHAO, JIANG QIANG, ZHONG JIFENG, HUANG JINMING, WANG XIUGE, ZHANG YARAN, GAO YAPING, JU ZHIHUA, GAO YUNDONG
• Format: Patent
• Language: Chinese; English
• Published: 29.01.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; SPIRITS; TESTING; VINEGAR; WINE

The invention provides a method for detecting different beta-casein variants in milk. The method comprises the following steps: S1. extracting proteins from a to-be-detected sample; S2. performing enzymatic digestion on the extracted proteins by adopting trypsin and V8 protease; and S3. performing mass spectrometry detection on peptide fragments generated by protein enzymolysis to identify the beta-casein variants. The detection method disclosed by the invention is reasonable in design and can effectively detect different beta-casein variants (A1, A2, A3, B, C, E, F, H1 and I) in a dairy product, and is high in detection accuracy and easy to operate. The method can judge A2 type beta-casein variants of a terminal dairy product of a cow, and can regulate the A2 milk dairy product market. The method is a good application to a protein detection technology in production practice and can generate enormous economic and social benefits. 本发明提供种检测牛乳中不同β-酪蛋白变体型的方法，所述方法包括：S1.对待测样品进行蛋白质提取；S2.采用胰蛋白酶和V8蛋白酶对提取的蛋白质进行酶解消化；S3.