Preparation method and applications of secondary metabolites with antibacterial and/or antioxidant activity of casuarina equisetifolia endophytic fungi
The invention discloses a preparation method and applications of secondary metabolites with the antibacterial and/or antioxidant activity of casuarina equisetifolia endophytic fungi. The method comprises the following steps: (1) separating endophytic fungi from casuarina equisetifolia by adopting a...
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Main Authors | , , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
29.01.2019
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses a preparation method and applications of secondary metabolites with the antibacterial and/or antioxidant activity of casuarina equisetifolia endophytic fungi. The method comprises the following steps: (1) separating endophytic fungi from casuarina equisetifolia by adopting a tissue block separating method; (2) fermenting the separated endophytic fungi in a rice solid medium; (3) after fermentation is finished, carrying out cold soaking extraction on the fermentation product by adopting a mixed solution of ethyl acetate and acetone, and carrying out concentration under reduced pressure on the extracted solution, thus obtaining the secondary metabolites of the casuarina equisetifolia endophytic fungi; and (4) screening the secondary metabolites with the antibacterialand/or antioxidant activity by adopting the thin layer chromatography-bioautography method. According to the method, the secondary metabolites with the antibacterial and/or antioxidant activity can beobtained from the casuari |
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Bibliography: | Application Number: CN201811237375 |